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Biotechnology

Meeting the Diverse and Demanding Needs of Global Biotechs

Microfluidizer® Processing in the Biotechnology Industry

Biotech companies worldwide rely on Microfluidizer® fluid processing biotechnology equipment for successful cell homogenization. We offer high shear cell disruption, or cell lysis, capabilities with improved protein recovery and guaranteed scaleup. From the gentle disruption of cultured cells for virus isolation to the challenging rupture of yeast and other fungi, Microfluidizer® homogenizer technology enables leading biotech firms (including four of the top five) to meet their fluid processing needs.

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Tough on Cells, Gentle on Proteins

The flexible, robust Microfluidizer® provides better processing results than can be attained from other cell disruption methods or technologies. Our high-pressure homogenizers are used to rupture a wide variety of cells with specific shear requirements. By precisely controlling shear, our customers are able to use the lowest pressure possible to achieve target cell rupture rates. In addition, Microfluidizer® technology requires fewer passes and effectively cools the product with a heat exchanger. All of these factors combine to ensure maximum cell breakage and protein harvesting.

Biotechnology Applications

  • E. coli
  • Yeast cells
  • Algae
  • Mammalian tissue
  • Bacteria
  • Plant
  • Insect
  • Fungi

Technical Features

  • High shear particle size reduction
  • Uniform shear rates
  • Rapid cell rupturing
  • Ease of use
  • Simple cleaning process
  • Biopharma models available
  • Effective product cooling
  • Small sample volumes

Benefits to Pharmaceuticals

  • Efficient cell disintegration
  • High protein recovery
  • Scalable
  • Fewer passes required
  • Less time to operate and clean
  • Repeatable results
  • Supports multiple applications
  • Faster, simpler filtering

Proven Processing Results

In E. coli and yeast applications, rupture rates of greater than 99% have been achieved with Microfluidizer® technology. In addition, we work with customers to ensure that the optimal pressure is used for each application to balance the rupture rate with maximum recovery of soluble protein. In the case of lysis of haploid S. Pombe, we determined that five passes was optimal.

E. Coli Cell Rupture
BeforeAfter

 

Yeast Lysis (S. Pombe)
Un-lysed5 Passes

 

 

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